This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCerulean3-Bcl2-pEGFP-C1
catalog :
177416
citations: 1
product information
Catalog Number :
177416
Product Name :
mCerulean3-Bcl2-pEGFP-C1
article :
| doi | 10.7554/eLife.37689 |
| id | 28216034 |
| pubmed_id | 30860026 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pEGFP-C1 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Transfection of this plasmid will express mCerulean3-fused to the N-terminus of Bcl-2. There is a flexible, 7 aa linker SGLRSTR , between mCerulean3 and Bcl2.
growth strain :
To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2.
origin :
37
pi :
|
plasmid copy :
Human Bcl2 sequence aligns with Genebank accession # M14745.1, except there is a M156I alteration in the coding sequence. The mutation was in the original clone (Janiak et al., 1994) published in JBC, and has been used to inhibit apoptosis by our group and many others. It has always been our assumption that this mutation is a naturally occurring variant. Our clone has been used in many publications as the plasmid was widely distributed when it was first assembled and published. Janiak F, Leber B, Andrews DW. Assembly of Bcl-2 into microsomal and outer mitochondrial membranes. J Biol Chem. 1994 Apr 1;269(13):9842-9. PMID: 8144576. mCerulean3 sequence received from Mark Rizzo PMID: 21479270.
resistance markers :
| 4132 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments