This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCherry-7aa-ActA-pEGFP-C1
catalog :
177406
citations: 1
Reference
Aranovich A, Liu Q, Collins T, Geng F, Dixit S, Leber B, et al. Differences in the mechanisms of proapoptotic BH3 proteins binding to Bcl-XL and Bcl-2 quantified in live MCF-7 cells. Mol Cell. 2012;45:754-63 pubmed publisher
product information
Catalog Number :
177406
Product Name :
mCherry-7aa-ActA-pEGFP-C1
article :
doi10.1016/j.molcel.2012.01.030
id28223013
pubmed_id22464442
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Transfection of this plasmid will express mCherry-fused to the N-terminus of ActA tail anchor sequence: LILAMLAIGVFSLGAFIKIIQLRKNN , which targets the mitochondrial outer membrane and signal correlates with mitotracker Green signal in cells. 7aa indicates the 7 aa flexible linker region, SGLRSRV between mCherry and the ActA targeting signal.
growth strain :
To express mCherry fused to mitochondria outer membrane targeting sequence ActA . mCherry faces the cytosol.
origin :
37
pi :
alt_names
mCherry-ActA, Ch-ActA, ChActA
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseslmo0204
geneactA
id987035
genbank_ids
mutation
nameActA tail anchor
shRNA_sequence
size
species
32644
Other
Listeria monocytogenes
tags
locationN terminal on insert
tagmCherry
plasmid copy :
See gene entry for actin assembly-inducing protein ActA (NCBI Reference Sequence: WP_110138194.1). Here we have used only the C-terminal tail anchor sequence of this protein. mCherry sequence matches GenBank: AZP55984.1.
resistance markers :
4132
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA