This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Venus-7aa-ActA-pEGFP-C1
catalog :
177405
product information
Catalog Number :
177405
Product Name :
Venus-7aa-ActA-pEGFP-C1
article :
doi10.21203/rs.3.pex-1354/v1
id28233208
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Transfection of this plasmid will express Venus-fused to the N-terminus of ActA tail anchor sequence: LILAMLAIGVFSLGAFIKIIQLRKNN , which targets the mitochondrial outer membrane and signal correlates with mitotracker Red signal in cells. 7aa indicates the 7 aa flexible linker region, SGLRSRV between Venus and the ActA targeting signal. This Venus protein contains an F224R mutation to make it monomeric.
growth strain :
To express Venus fused to mitochondria outer membrane targeting sequence ActA . Venus faces the cytosol.
origin :
37
pi :
alt_names
Venus-ActA, V-ActA, VActA
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseslmo0204
geneactA
id987035
genbank_ids
mutation
nameActA tail anchor
shRNA_sequence
size
species
32644
Other
Listeria monocytogenes
tags
locationN terminal on insert
tagVenus
plasmid copy :
See gene entry for actin assembly-inducing protein ActA (NCBI Reference Sequence: WP_110138194.1). Here we have used only the C-terminal tail anchor sequence of this protein. Venus sequence received from Dr. Ray Truant (McMaster University).
resistance markers :
4132
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA