pAAV-CAG-H2BmGreenLantern | Addgene 177332 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-CAG-H2BmGreenLantern

catalog :

177332

citations: 1

Reference
Wang Z, Romanski A, Mehra V, Wang Y, Brannigan M, Campbell B, et al. Brain-wide analysis of the supraspinal connectome reveals anatomical correlates to functional recovery after spinal injury. elife. 2022;11: pubmed publisher

product information

Catalog Number :

177332

Product Name :

pAAV-CAG-H2BmGreenLantern

article :

doi	10.7554/eLife.76254
id	28229396
pubmed_id	35838234

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV-CAG-GFP

backbone_mutation

The GFP of the original vector was excised with XbaI and XhoI and replaced with the H2BmGL

backbone_origin

Boyden laboratory

backbone_size

4714

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

growth notes :

For more information, also see Campbell et al. 2020 mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging PNAS, https://doi.org/10.1073/pnas.2000942117.

growth strain :

To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	XbaI or HindIII
promoter	CAG
sequencing_primer_3	WPRE-R
sequencing_primer_5	pCAG-F or pCAGGS-5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Hist1h2bh
gene	Hist1h2bq
id	306945

genbank_ids

NP_001100822

mutation

This fusion protein was optimized to the Human codon usage.

name

H2B

shRNA_sequence

size

1144

species

10116

Rattus norvegicus

32644

Other

Rat