This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF1-Hs Cdk1/cyclin B1/Cks1
catalog :
177329
citations: 1
Reference
Yu J, Raia P, Ghent C, Raisch T, Sadian Y, Cavadini S, et al. Structural basis of human separase regulation by securin and CDK1-cyclin B1. Nature. 2021;596:138-142 pubmed publisher
product information
Catalog Number :
177329
Product Name :
pF1-Hs Cdk1/cyclin B1/Cks1
article :
doi10.1038/s41586-021-03764-0
id28222995
pubmed_id34290405
bacterial resistance :
Ampicillin
cloning :
backbonepF1
backbone_mutation
backbone_origin
backbone_size6424
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Genes are gene-optimised for insect cell expression and have been ordered from GeneArts.
growth strain :
Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex
origin :
37
pi :
alt_names
CDK1
CDC2
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpolh
sequencing_primer_3CATCTTCTTGATCTGGTTGT
sequencing_primer_5GAAGATTACACCAAGATCGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDC2, CDC28A, P34CDC2
geneCDK1
id983
genbank_ids
CAA28963.1
mutation
nameCyclin-dependent kinase 1
shRNA_sequence
size891
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag8 x His
alt_names
CCNB1
cyclin B1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterp10
sequencing_primer_3GCTCTGCGTGTGACCCGTAA
sequencing_primer_5CACCTTAGCCACAGCCTTAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCNB
geneCCNB1
id891
genbank_ids
BAF82120.1
mutation
nameG2/mitotic-specific cyclin-B1
shRNA_sequence
size1299
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag2 x Strep-tag
alt_names
CKS1B
CKS1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterp10
sequencing_primer_3TCCCACAAGCAGATCTACTA
sequencing_primer_5CTTCTTGGGCTTCTTAGGCA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCKS1, PNAS-16, PNAS-18, ckshs1
geneCKS1B
id1163
genbank_ids
CAA38702.1
mutation
nameCyclin-dependent kinases regulatory subunit 1
shRNA_sequence
size240
species
9606
Homo sapiens
tags
resistance markers :
5694
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA