pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D | Addgene 177320 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

catalog :

177320

citations: 1

Reference
Nan X, TamgÃ¼ney T, Collisson E, Lin L, Pitt C, Galeas J, et al. Ras-GTP dimers activate the Mitogen-Activated Protein Kinase (MAPK) pathway. Proc Natl Acad Sci U S A. 2015;112:7996-8001 pubmed publisher

product information

Catalog Number :

177320

Product Name :

pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

article :

doi	10.1073/pnas.1509123112
id	16838
pubmed_id	26080442

bacterial resistance :

Ampicillin

cloning :

backbone

pLenti-TO/CMV-puro

backbone_mutation

backbone_origin

Addgene

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The lentiviral backbone was created by Eric Campeau et al. (https://www.addgene.org/17293/) as a DESTination vector. The full insert was first created in an ENTRY clone (Invitrogen) and transferred to the DEST vector via gateway cloning.

growth strain :

Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

origin :

pi :

alt_names

KRAS2

KRAS

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	TetOn-CMV
sequencing_primer_3
sequencing_primer_5	CMV_Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
gene	KRAS
id	3845

genbank_ids

mutation

G12D

name

KRAS 4B

shRNA_sequence

size

567

species

9606

Homo sapiens