This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKM631
catalog :
17727
citations: 1
Reference
Kapust R, Tozser J, Copeland T, Waugh D. The P1' specificity of tobacco etch virus protease. Biochem Biophys Res Commun. 2002;294:949-55 pubmed
product information
Catalog Number :
17727
Product Name :
pKM631
article :
doi10.1016/S0006-291X(02)00574-0
id2029
pubmed_id12074568
bacterial resistance :
Ampicillin
cloning :
backbonepKM596
backbone_mutation
backbone_originWaugh lab
backbone_size8264
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This vector produces an MBP-NusG-His6 fusion protein with a recognition site for TEV protease (ENLYFQG) between MBP and NusG-His6. See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.
origin :
37
pi :
alt_names
N-utilization substance G
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genenusG
id1193654
genbank_ids
NC_000918
mutation
nameNusG
shRNA_sequence
size747
species
Aquifex aeolicus
tags
locationN terminal on backbone
tagMBP
locationC terminal on insert
tagHis6
resistance markers :
565
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA