This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTPTP alpha
catalog :
17691
citations: 1
| Reference |
|---|
Zheng X, Resnick R, Shalloway D. A phosphotyrosine displacement mechanism for activation of Src by PTPalpha. EMBO J. 2000;19:964-78 pubmed
|
product information
Catalog Number :
17691
Product Name :
pTPTP alpha
article :
| doi | 10.1093/emboj/19.5.964 |
| id | 2022 |
| pubmed_id | 10698938 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pTet-splice | |
| backbone_mutation | ||
| backbone_origin | Invitrogen | |
| backbone_size | 5178 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGCACGTCGTATGGGTACTTGAAGT- TGGCATAATCTGA-3'. The 5' primer contains a HindIII site, a Kozak consensus sequence CCACC and the start codon. The 3' primer contains the coding sequence for the HA epitope YPYDVPDYA, the stop codon and a HindIII site. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk (Life Technologies), it expresses protein only in the absence of doxycycline.
5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTC
TTGT-3'
PTPalpha-HA was amplified from plasmid pXJ41PTPalpha using the 5' primer
5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTC
TTGT-3'
PTPalpha-HA was amplified from plasmid pXJ41PTPalpha using the 5' primer
origin :
37
pi :
|
resistance markers :
| 614 |
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments