This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pYESir2-puro
catalog :
1769
citations: 3
Reference
Latifkar A, Ling L, Hingorani A, Johansen E, Clement A, Zhang X, et al. Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity. Dev Cell. 2019;49:393-408.e7 pubmed publisher
Jang K, Noh S, Lehwald N, Tao G, Bellovin D, Park H, et al. SIRT1 and c-Myc promote liver tumor cell survival and predict poor survival of human hepatocellular carcinomas. PLoS ONE. 2012;7:e45119 pubmed
Vaziri H, Dessain S, Ng Eaton E, Imai S, Frye R, Pandita T, et al. hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Cell. 2001;107:149-59 pubmed
product information
Catalog Number :
1769
Product Name :
pYESir2-puro
article :
doi10.1016/S0092-8674(01)00527-X
id1
pubmed_id11672523
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
SspI site in Amp gene.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaBI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2, SIR2L1, SIR2alpha
geneSIRT1
id23411
genbank_ids
mutation
nameSir2
shRNA_sequence
size2500
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA