pcAla12 | Addgene 17680 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcAla12

catalog :

17680

citations: 1

Reference
Yaciuk P, Choi J, Shalloway D. Mutation of amino acids in pp60c-src that are phosphorylated by protein kinases C and A. Mol Cell Biol. 1989;9:2453-63 pubmed

product information

Catalog Number :

17680

Product Name :

pcAla12

article :

doi
id	2018
pubmed_id	2474754

bacterial resistance :

Ampicillin

cloning :

backbone

pEVX

backbone_mutation

backbone_origin

backbone_size

6400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The pM5HHB5 c-src sequence between nucleotide 1 (numbering from the c-src translational initiator ATG) and nucleotide 43 (at an NaeI site) was replaced with the sequence ATGGGGAGTAGCAAGAGCAAGCCTAAGGATC CCGCTCAGCGCC in pcAlal2. This introduced MstII and BamHI restriction sites near nucleotides 25 and 29, respectively, and changed the AGC (Ser) 12 codon to GCT (Ala) codon yet preserved the remaining the c-src amino acid sequence.

origin :

pi :

alt_names

c-Src

src

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	PP60C-SCR, SDR, c-src
gene	SRC
id	396442

genbank_ids

mutation

Ser 12 changed to Ala.

name

c-src S12A

shRNA_sequence

size

species

9031

Gallus gallus