This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe-puro Ras V12
catalog :
1768
citations: 22
Reference
Mart xed nez Zamudio R, Stefa A, Nabuco Leva Ferreira Freitas J, Vasilopoulos T, Simpson M, Dor xe9 G, et al. Escape from oncogene-induced senescence is controlled by POU2F2 and memorized by chromatin scars. Cell Genom. 2023;3:100293 pubmed publisher
Reisman B, Guo H, Ramsey H, Wright M, Reinfeld B, Ferrell P, et al. Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase. Nat Chem Biol. 2021;: pubmed publisher
Mangialardi E, Chen K, Salmon B, Vacher J, Salmena L. Investigating the duality of Inpp4b function in the cellular transformation of mouse fibroblasts. Oncotarget. 2019;10:6378-6390 pubmed publisher
Razdan N, Vasilopoulos T, Herbig U. Telomere dysfunction promotes transdifferentiation of human fibroblasts into myofibroblasts. Aging Cell. 2018;17:e12838 pubmed publisher
Kim Y, Um J, Yun J. A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence. J Vis Exp. 2018;: pubmed publisher
Yang Z, Shah K, Busby T, Giles K, Khodadadi Jamayran A, Li W, et al. Hijacking a key chromatin modulator creates epigenetic vulnerability for MYC-driven cancer. J Clin Invest. 2018;128:3605-3618 pubmed publisher
Poli V, Fagnocchi L, Fasciani A, Cherubini A, Mazzoleni S, Ferrillo S, et al. MYC-driven epigenetic reprogramming favors the onset of tumorigenesis by inducing a stem cell-like state. Nat Commun. 2018;9:1024 pubmed publisher
Giuliano C, Lin A, Smith J, Palladino A, Sheltzer J. MELK expression correlates with tumor mitotic activity but is not required for cancer growth. elife. 2018;7: pubmed publisher
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
Dang H, Takai A, Forgues M, Pomyen Y, Mou H, Xue W, et al. Oncogenic Activation of the RNA Binding Protein NELFE and MYC Signaling in Hepatocellular Carcinoma. Cancer Cell. 2017;32:101-114.e8 pubmed publisher
Patel P, Suram A, Mirani N, Bischof O, Herbig U. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc Natl Acad Sci U S A. 2016;113:E5024-33 pubmed publisher
Kümper S, Mardakheh F, McCarthy A, Yeo M, Stamp G, Paul A, et al. Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis. elife. 2016;5:e12994 pubmed publisher
Pelletier J, Roux D, Viollet B, Mazure N, Pouysségur J. AMP-activated protein kinase is dispensable for maintaining ATP levels and for survival following inhibition of glycolysis, but promotes tumour engraftment of Ras-transformed fibroblasts. Oncotarget. 2015;6:11833-47 pubmed
Leiser D, Medová M, Mikami K, Nisa L, Stroka D, Blaukat A, et al. KRAS and HRAS mutations confer resistance to MET targeting in preclinical models of MET-expressing tumor cells. Mol Oncol. 2015;9:1434-46 pubmed publisher
Guo J, Wang Y, Sachs F, Meng F. Actin stress in cell reprogramming. Proc Natl Acad Sci U S A. 2014;111:E5252-61 pubmed publisher
Becker L, Takwi A, Lu Z, Li Y. The role of miR-200a in mammalian epithelial cell transformation. Carcinogenesis. 2015;36:2-12 pubmed publisher
Cao X, Kong C, Mathi K, Lim Y, Cacheux Rataboul V, Wang X. The use of transformed IMR90 cell model to identify the potential extra-telomeric effects of hTERT in cell migration and DNA damage response. BMC Biochem. 2014;15:17 pubmed publisher
Gohar A, Cao R, Jenkins P, Li W, Houston J, Houston K. Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry. Biomed Opt Express. 2013;4:1390-400 pubmed publisher
Jackstadt R, Jung P, Hermeking H. AP4 directly downregulates p16 and p21 to suppress senescence and mediate transformation. Cell Death Dis. 2013;4:e775 pubmed publisher
Cipriano R, Miskimen K, Bryson B, Foy C, Bartel C, Jackson M. FAM83B-mediated activation of PI3K/AKT and MAPK signaling cooperates to promote epithelial cell transformation and resistance to targeted therapies. Oncotarget. 2013;4:729-38 pubmed
Junk D, Cipriano R, Stampfer M, Jackson M. Constitutive CCND1/CDK2 activity substitutes for p53 loss, or MYC or oncogenic RAS expression in the transformation of human mammary epithelial cells. PLoS ONE. 2013;8:e53776 pubmed publisher
Suram A, Kaplunov J, Patel P, Ruan H, Cerutti A, Boccardi V, et al. Oncogene-induced telomere dysfunction enforces cellular senescence in human cancer precursor lesions. EMBO J. 2012;31:2839-51 pubmed publisher
product information
Catalog Number :
1768
Product Name :
pBabe-puro Ras V12
article :
doi
id102
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
origin :
37
pi :
alt_names
HrasV12
Hras
v-Ha-ras
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationG12V, constitutively active
nameras V12
shRNA_sequence
size700
species
9606
Homo sapiens
tags
plasmid copy :
Scott Lowe
resistance markers :
568
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA