This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMcSrc
catalog :
17672
citations: 2
Reference
Pal R, Bajaj L, Sharma J, Palmieri M, di Ronza A, Lotfi P, et al. NADPH oxidase promotes Parkinsonian phenotypes by impairing autophagic flux in an mTORC1-independent fashion in a cellular model of Parkinson's disease. Sci Rep. 2016;6:22866 pubmed publisher
Johnson P, Coussens P, Danko A, Shalloway D. Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells. Mol Cell Biol. 1985;5:1073-83 pubmed
product information
Catalog Number :
17672
Product Name :
pMcSrc
article :
doi
id2015
pubmed_id2582237
bacterial resistance :
Ampicillin
cloning :
backbonepEVX
backbone_mutation
backbone_origin
backbone_size6400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pMcsrc was constructed by gel purifying the 8.1-kilobase (kb) BamHI c-src fragment running from c-src coordinates -2.1 to 6.0 kb and ligating it into plasmid pEVX which had been linearized at a unique BglII site lying between the two Moloney murine leukemia virus (MoMLV) long terminal repeats (LTRs). The upstream BamHI site is about 270 base pairs (bp) upstream from the beginning of c-src exon no. 2, whereas the downstream site lies about 1.2 kb beyond the c-src termination codon.
origin :
37
pi :
alt_names
c-Src
src
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI / BglII
cloning_site_5BamHI / BglII
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesPP60C-SCR, SDR, c-src
geneSRC
id396442
genbank_ids
mutation
namec-src
shRNA_sequence
size8100
species
9031
Gallus gallus
tags
resistance markers :
460
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA