This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE-puro-GFP-progerin
catalog :
17663
citations: 15
Reference
Ziegler D, Czarnecka Herok J, Vernier M, Scholtes C, Camprubi C, Huna A, et al. Cholesterol biosynthetic pathway induces cellular senescence through ERRα. NPJ Aging. 2024;10:5 pubmed publisher
Herrera I, Fernandes J, Shir Mohammadi K, Levesque J, Mattar P. Lamin A upregulation reorganizes the genome during rod photoreceptor degeneration. Cell Death Dis. 2023;14:701 pubmed publisher
Coll Bonfill N, Mahajan U, Shashkova E, Lin C, Mecham R, Gonzalo S. Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature. GeroScience. 2022;: pubmed publisher
Jiang Y, Ji J. Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells. Front Aging. 2022;3:844885 pubmed publisher
Bin Imtiaz M, Royall L, Gonzalez Bohorquez D, Jessberger S. Human neural progenitors establish a diffusion barrier in the endoplasmic reticulum membrane during cell division. Development. 2022;149: pubmed publisher
VON KLEECK R, Castagnino P, Assoian R. Progerin mislocalizes myocardin-related transcription factor in Hutchinson-Guilford Progeria syndrome. Vasc Biol. 2022;4:1-10 pubmed publisher
Sanders J, Golloshi R, Das P, Xu Y, Terry P, Nash D, et al. Loops, topologically associating domains, compartments, and territories are elastic and robust to dramatic nuclear volume swelling. Sci Rep. 2022;12:4721 pubmed publisher
Han M, Pandey D. ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial Plasminogen Activator Inhibitor-1. Am J Respir Cell Mol Biol. 2021;: pubmed publisher
Machiela E, Jeloka R, Caron N, Mehta S, Schmidt M, Baddeley H, et al. The Interaction of Aging and Cellular Stress Contributes to Pathogenesis in Mouse and Human Huntington Disease Neurons. Front Aging Neurosci. 2020;12:524369 pubmed publisher
Choi H, Kim T, Jeong J, Strandgren C, Eriksson M, Cho E. Expression of the Hutchinson-Gilford Progeria Mutation Leads to Aberrant Dentin Formation. Sci Rep. 2018;8:15368 pubmed publisher
Griveau A, Wiel C, Le Calve B, Ziegler D, Djebali S, Warnier M, et al. Targeting the phospholipase A2 receptor ameliorates premature aging phenotypes. Aging Cell. 2018;17:e12835 pubmed publisher
Pacheco L, Gomez L, Dias J, Ziebarth N, Howard G, Schiller P. Progerin expression disrupts critical adult stem cell functions involved in tissue repair. Aging (Albany NY). 2014;6:1049-63 pubmed
Villa Bellosta R, Rivera Torres J, Osorio F, Acin Perez R, EnrĂ­quez J, Lopez Otin C, et al. Defective extracellular pyrophosphate metabolism promotes vascular calcification in a mouse model of Hutchinson-Gilford progeria syndrome that is ameliorated on pyrophosphate treatment. Circulation. 2013;127:2442-51 pubmed publisher
Liu G, Barkho B, Ruiz S, Diep D, Qu J, Yang S, et al. Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome. Nature. 2011;472:221-5 pubmed publisher
Scaffidi P, Misteli T. Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nat Cell Biol. 2008;10:452-9 pubmed publisher
product information
Catalog Number :
17663
Product Name :
pBABE-puro-GFP-progerin
article :
doi10.1038/ncb1708
id2011
pubmed_id18311132
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
This vector was generated by cloning the NheI BamHI fragment from pEGFP D50 lamin A into the BamHI site of the pBABE-puro vector after filling of the ends.
origin :
37
pi :
alt_names
lamin A
progerin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
geneLMNA
id4000
genbank_ids
mutationDelta 50
nameGFP-D50 lamin A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
343
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA