This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE-puro-GFP-wt-lamin A
catalog :
17662
citations: 24
Reference
Ziegler D, Czarnecka Herok J, Vernier M, Scholtes C, Camprubi C, Huna A, et al. Cholesterol biosynthetic pathway induces cellular senescence through ERRα. NPJ Aging. 2024;10:5 pubmed publisher
Herrera I, Fernandes J, Shir Mohammadi K, Levesque J, Mattar P. Lamin A upregulation reorganizes the genome during rod photoreceptor degeneration. Cell Death Dis. 2023;14:701 pubmed publisher
Zhang N, Hu Q, Sui T, Fu L, Zhang X, Wang Y, et al. Unique progerin C-terminal peptide ameliorates Hutchinson-Gilford progeria syndrome phenotype by rescuing BUBR1. Nat Aging. 2023;3:185-201 pubmed publisher
Jiang Y, Ji J. Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells. Front Aging. 2022;3:844885 pubmed publisher
Rubio Ramos A, Bernabé Rubio M, Labat de Hoz L, Casares Arias J, Kremer L, Correas I, et al. MALL, a membrane-tetra-spanning proteolipid overexpressed in cancer, is present in membraneless nuclear biomolecular condensates. Cell Mol Life Sci. 2022;79:236 pubmed publisher
Sanders J, Golloshi R, Das P, Xu Y, Terry P, Nash D, et al. Loops, topologically associating domains, compartments, and territories are elastic and robust to dramatic nuclear volume swelling. Sci Rep. 2022;12:4721 pubmed publisher
John S, K G G, Krishna A, Mishra R. Neurotherapeutic implications of sense and respond strategies generated by astrocytes and astrocytic tumours to combat pH mechanical stress. Neuropathol Appl Neurobiol. 2021;: pubmed publisher
Tamura R, Jiang F, Xie J, Kamiyama D. Multiplexed labeling of cellular proteins with split fluorescent protein tags. Commun Biol. 2021;4:257 pubmed publisher
Young A, Gunn A, Hatch E. BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins. Mol Biol Cell. 2020;31:1551-1560 pubmed publisher
Dunlevy K, Medvedeva V, Wilson J, Hoque M, Pellegrin T, Maynard A, et al. The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting. J Cell Sci. 2020;133: pubmed publisher
Schormann W, Hariharan S, Andrews D. A reference library for assigning protein subcellular localizations by image-based machine learning. J Cell Biol. 2020;219: pubmed publisher
Botton T, Talevich E, Mishra V, Zhang T, Shain A, Berquet C, et al. Genetic Heterogeneity of BRAF Fusion Kinases in Melanoma Affects Drug Responses. Cell Rep. 2019;29:573-588.e7 pubmed publisher
Choi H, Kim T, Jeong J, Strandgren C, Eriksson M, Cho E. Expression of the Hutchinson-Gilford Progeria Mutation Leads to Aberrant Dentin Formation. Sci Rep. 2018;8:15368 pubmed publisher
Griveau A, Wiel C, Le Calve B, Ziegler D, Djebali S, Warnier M, et al. Targeting the phospholipase A2 receptor ameliorates premature aging phenotypes. Aging Cell. 2018;17:e12835 pubmed publisher
Zhang Y, Wang J, Huang W, Cai J, Ba J, Wang Y, et al. Nuclear Nestin deficiency drives tumor senescence via lamin A/C-dependent nuclear deformation. Nat Commun. 2018;9:3613 pubmed publisher
Infante E, Castagnino A, Ferrari R, Monteiro P, Agüera González S, Paul Gilloteaux P, et al. LINC complex-Lis1 interplay controls MT1-MMP matrix digest-on-demand response for confined tumor cell migration. Nat Commun. 2018;9:2443 pubmed publisher
Bartova E, Legartova S, Krejci J, Řezníčková P, Kovaříková A, Suchankova J, et al. Depletion of A-type lamins and Lap2? reduces 53BP1 accumulation at UV-induced DNA lesions and Lap2? protein is responsible for compactness of irradiated chromatin. J Cell Biochem. 2018;: pubmed publisher
Barilari M, Bonfils G, Treins C, Koka V, De Villeneuve D, Fabrega S, et al. ZRF1 is a novel S6 kinase substrate that drives the senescence programme. EMBO J. 2017;36:736-750 pubmed publisher
Pacheco L, Gomez L, Dias J, Ziebarth N, Howard G, Schiller P. Progerin expression disrupts critical adult stem cell functions involved in tissue repair. Aging (Albany NY). 2014;6:1049-63 pubmed
Sehnalová P, Legartova S, Cmarko D, Kozubek S, Bartova E. Recruitment of HP1? to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins. Biol Cell. 2014;106:151-65 pubmed publisher
Mahen R, Hattori H, Lee M, Sharma P, Jeyasekharan A, Venkitaraman A. A-type lamins maintain the positional stability of DNA damage repair foci in mammalian nuclei. PLoS ONE. 2013;8:e61893 pubmed publisher
Stixova L, Bartova E, Matula P, Danek O, Legartova S, Kozubek S. Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin. Epigenetics Chromatin. 2011;4:5 pubmed publisher
Liu G, Barkho B, Ruiz S, Diep D, Qu J, Yang S, et al. Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome. Nature. 2011;472:221-5 pubmed publisher
Scaffidi P, Misteli T. Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nat Cell Biol. 2008;10:452-9 pubmed publisher
product information
Catalog Number :
17662
Product Name :
pBABE-puro-GFP-wt-lamin A
article :
doi10.1038/ncb1708
id2011
pubmed_id18311132
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
This vector was generated by cloning the NheI BamHI fragment from pEGFP wt-lamin A into the BamHI site of the pBABE-puro vector after filling of the ends.
origin :
37
pi :
alt_names
lamin A
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
geneLMNA
id4000
genbank_ids
mutation
nameGFP-lamin A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
343
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA