This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ISceI-GR-RFP
catalog :
17654
citations: 15
Reference
Tsuji K, Kikuchi E, Takashima Y, Shoji T, Takahashi H, Ito S, et al. Inhibition of non-homologous end joining mitigates paclitaxel resistance resulting from mitotic slippage in non-small cell lung cancer. Cell Cycle. 2023;22:1854-1864 pubmed publisher
RICHARDS F, Llorca Cardeñosa M, Langton J, Buch Larsen S, Shamkhi N, Sharma A, et al. Regulation of Rad52-dependent replication fork recovery through serine ADP-ribosylation of PolD3. Nat Commun. 2023;14:4310 pubmed publisher
Ghosh I, Kwon Y, Shabestari A, Chikhale R, Chen J, Wiese C, et al. TLK1-mediated RAD54 phosphorylation spatio-temporally regulates Homologous Recombination Repair. Nucleic Acids Res. 2023;: pubmed publisher
Kriger D, Novitskaya K, Vasileva G, Lomert E, Aksenov N, Barlev N, et al. Alpha-actnin-4 (ACTN4) selectively affects the DNA double-strand breaks repair in non-small lung carcinoma cells. Biol Direct. 2022;17:40 pubmed publisher
Khozooei S, Lettau K, Barletta F, Jost T, Rebholz S, Veerappan S, et al. Fisetin induces DNA double-strand break and interferes with the repair of radiation-induced damage to radiosensitize triple negative breast cancer cells. J Exp Clin Cancer Res. 2022;41:256 pubmed publisher
Marzio A, Kurz E, Sahni J, Di Feo G, Puccini J, Jiang S, et al. EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion. Cell. 2022;185:169-183.e19 pubmed publisher
Llorens Agost M, Ensminger M, Le H, Gawai A, Liu J, Cruz García A, et al. POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis. Nat Cell Biol. 2021;23:1095-1104 pubmed publisher
Yang G, Chen Y, Wu J, Chen S, Liu X, Singh A, et al. Poly(ADP-ribosyl)ation mediates early phase histone eviction at DNA lesions. Nucleic Acids Res. 2020;48:3001-3013 pubmed publisher
Takashima Y, Kikuchi E, Kikuchi J, Suzuki M, Kikuchi H, Maeda M, et al. Bromodomain and extraterminal domain inhibition synergizes with WEE1-inhibitor AZD1775 effect by impairing nonhomologous end joining and enhancing DNA damage in nonsmall cell lung cancer. Int J Cancer. 2019;: pubmed publisher
Vítor A, Sridhara S, Sabino J, Afonso A, Grosso A, Martin R, et al. Single-molecule imaging of transcription at damaged chromatin. Sci Adv. 2019;5:eaau1249 pubmed publisher
Róna G, Roberti D, Yin Y, Pagan J, Homer H, Sassani E, et al. PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading. elife. 2018;7: pubmed publisher
Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, et al. Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nat Cell Biol. 2017;19:1400-1411 pubmed publisher
Vazquez B, Thackray J, Simonet N, Kane Goldsmith N, Martínez Redondo P, Nguyen T, et al. SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair. EMBO J. 2016;35:1488-503 pubmed publisher
Lin J, Chen H, Luo L, Lai Y, Xie W, Kee K. Creating a monomeric endonuclease TALE-I-SceI with high specificity and low genotoxicity in human cells. Nucleic Acids Res. 2015;43:1112-22 pubmed publisher
Soutoglou E, Dorn J, Sengupta K, Jasin M, Nussenzweig A, Ried T, et al. Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol. 2007;9:675-82 pubmed
product information
Catalog Number :
17654
Product Name :
ISceI-GR-RFP
article :
doi10.1038/ncb1591
id2009
pubmed_id17486118
bacterial resistance :
Kanamycin
cloning :
backbonepDsRed-Monomer-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The Ligand Binding Domain (LBD) of GR was amplified from pCI-nGFP-C656G GR (gift from G. Hager) and cloned into pEGFP-C2 (Clontech) in the SacII and BamHI sites. The C656G mutation of GR that makes it more responsive to ligand and trasnfers it more quickly to the nucleus. Similarly, the I-SceI cDNA was amplified from HA-I-SceI and cloned into the previously described GFP-GRLBD into the SacI and SalI restriction sites. Finally, the chimeric I-SceI GR cDNA was subcloned into pDsRed-Monomer-C1 (Clontech) into the SacI and BamHI sites.
origin :
37
pi :
alt_names
SCEI
GR ligand binding domain
glucocorticoid receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SacI
promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5DsRed1-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesQ0160
geneSCEI
id854590
aliasesGR, Gcr, Grl
geneNr3c1
id24413
genbank_ids
mutationC655G mutation in the glucocorticoid receptor (see comments)
nameI-SceI-GRLBD
shRNA_sequence
size
species
10116
Rattus norvegicus
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagmRFP
resistance markers :
343
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA