This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-D50 lamin A
catalog :
17653
citations: 14
Reference
Batha L, Aziz M, Zhra M, Holail J, Al Qahtani W, Fakhoury R, et al. Differential Gene Expression Signatures and Cellular Signaling Pathways induced by Lamin A/C Transcript Variants in MCF7 Cell Line. Front Biosci (Landmark Ed). 2023;28:113 pubmed publisher
Joudeh L, DiCintio A, Ries M, Gasperson A, Griffin K, Robbins V, et al. Corruption of DNA end-joining in mammalian chromosomes by progerin expression. DNA Repair (Amst). 2023;126:103491 pubmed publisher
Sanders J, Golloshi R, Das P, Xu Y, Terry P, Nash D, et al. Loops, topologically associating domains, compartments, and territories are elastic and robust to dramatic nuclear volume swelling. Sci Rep. 2022;12:4721 pubmed publisher
Mohebi A, Le Gratiet A, Trianni A, Callegari F, Bianchini P, Diaspro A. Phasor map analysis to investigate Hutchinson-Gilford progeria cell under polarization-resolved optical scanning microscopy. Sci Rep. 2022;12:1679 pubmed publisher
Pack L, Daigh L, Chung M, Meyer T. Clinical CDK4/6 inhibitors induce selective and immediate dissociation of p21 from cyclin D-CDK4 to inhibit CDK2. Nat Commun. 2021;12:3356 pubmed publisher
Lionetti M, Bonfanti S, Fumagalli M, Budrikis Z, Font Clos F, Costantini G, et al. Chromatin and Cytoskeletal Tethering Determine Nuclear Morphology in Progerin-Expressing Cells. Biophys J. 2020;118:2319-2332 pubmed publisher
Tusamda Wakhloo N, Anders S, Badique F, Eichhorn M, Brigaud I, Petithory T, et al. Actomyosin, vimentin and LINC complex pull on osteosarcoma nuclei to deform on micropillar topography. Biomaterials. 2020;234:119746 pubmed publisher
Sarmento M, Oneto M, Pelicci S, Pesce L, Scipioni L, Faretta M, et al. Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures. Nat Commun. 2018;9:3415 pubmed publisher
Cho S, Abbas A, Irianto J, Ivanovska I, Xia Y, Tewari M, et al. Progerin phosphorylation in interphase is lower and less mechanosensitive than lamin-A,C in iPS-derived mesenchymal stem cells. Nucleus. 2018;9:230-245 pubmed publisher
Küchler P, Zimmermann G, Winzker M, Janning P, Waldmann H, Ziegler S. Identification of novel PDE? interacting proteins. Bioorg Med Chem. 2018;26:1426-1434 pubmed publisher
Piekarowicz K, Machowska M, Dratkiewicz E, Lorek D, Madej Pilarczyk A, Rzepecki R. The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells. Chromosoma. 2017;126:501-517 pubmed publisher
George C, Bozler J, Nguyen H, Bosco G. Condensins are Required for Maintenance of Nuclear Architecture. Cells. 2014;3:865-82 pubmed publisher
De Vos W, Houben F, Kamps M, Malhas A, Verheyen F, Cox J, et al. Repetitive disruptions of the nuclear envelope invoke temporary loss of cellular compartmentalization in laminopathies. Hum Mol Genet. 2011;20:4175-86 pubmed publisher
Scaffidi P, Misteli T. Reversal of the cellular phenotype in the premature aging disease Hutchinson-Gilford progeria syndrome. Nat Med. 2005;11:440-5 pubmed
product information
Catalog Number :
17653
Product Name :
pEGFP-D50 lamin A
article :
doi10.1038/nm1204
id2008
pubmed_id15750600
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4730
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
lamin A
progerin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SacII
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
geneLMNA
id4000
genbank_ids
mutationDelta 50 (50 aa internal deletion in globular tail domain)
nameD50 lamin A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
343
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA