This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PiggyBac-TetO-En1-t2a-Foxa2-hygro
catalog :
176483
citations: 1
Reference
Ng Y, Chanda S, Janas J, Yang N, Kokubu Y, Sudhof T, et al. Efficient generation of dopaminergic induced neuronal cells with midbrain characteristics. Stem Cell Reports. 2021;16:1763-1776 pubmed publisher
product information
Catalog Number :
176483
Product Name :
PiggyBac-TetO-En1-t2a-Foxa2-hygro
article :
doi10.1016/j.stemcr.2021.05.017
id28220192
pubmed_id34171286
bacterial resistance :
Ampicillin
cloning :
backbonePiggybac TetO - hygromycin
backbone_mutation
backbone_origin
backbone_size8621
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance
growth temp :
The Wernig lab uses Stbl3 or JM109 for downstream applications.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3PacI
cloning_site_5EcoRI
promoterTetO
sequencing_primer_3ggaggggcaaacaacagatg
sequencing_primer_5TGACCTCCATAGAAGACACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHNF-3-beta, HNF3B, TCF3B
geneFOXA2
id3170
aliasesEn-1, Mo-en.1, engrailed-1
geneEn1
id13798
genbank_ids
mutation
nameEn1-t2a-FoxA2
shRNA_sequence
size2673
species
9606
Homo sapiens
10090
Mus musculus
tags
resistance markers :
878
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA