This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2-MT GLI2 FL
catalog :
17648
citations: 16
Reference
Yang J, Wang J, Zhang Y, Huang W, Zhang S, Yin P, et al. c-Jun phosphorylated by JNK is required for protecting Gli2 from proteasomal-ubiquitin degradation by PGE2-JNK signaling axis. Biochim Biophys Acta Mol Cell Res. 2022;:119418 pubmed publisher
Swiderska Syn M, Mir Pedrol J, Oles A, Schleuger O, Salvador A, Greiner S, et al. Noncanonical activation of GLI signaling in SOX2+ cells drives medulloblastoma relapse. Sci Adv. 2022;8:eabj9138 pubmed publisher
Yang J, Wang J, Liu Y, Zhang Y, Huang W, Zou Y, et al. PGE2-JNK signaling axis non-canonically promotes Gli activation by protecting Gli2 from ubiquitin-proteasomal degradation. Cell Death Dis. 2021;12:707 pubmed publisher
Wang J, Zhang Y, Huang W, Yang J, Tang W, Huang T, et al. ABT-199 inhibits Hedgehog pathway by acting as a competitive inhibitor of oxysterol, rather as a BH3 mimetic. Acta Pharmacol Sin. 2020;: pubmed publisher
Xia L, Bouamar H, Gu X, Zeballos C, Qin T, Wang B, et al. Gli2 mediates the development of castration‑resistant prostate cancer. Int J Oncol. 2020;57:100-112 pubmed publisher
Valenza F, Cittaro D, Stupka E, Biancolini D, Patricelli M, Bonanomi D, et al. A novel truncating variant of GLI2 associated with Culler-Jones syndrome impairs Hedgehog signalling. PLoS ONE. 2019;14:e0210097 pubmed publisher
Babu D, Fanelli A, Mellone S, Muniswamy R, Wasniewska M, Prodam F, et al. Novel GLI2 mutations identified in patients with Combined Pituitary Hormone Deficiency (CPHD): Evidence for a pathogenic effect by functional characterization. Clin Endocrinol (Oxf). 2018;: pubmed publisher
Maiti S, Mondal S, Satyavarapu E, Mandal C. mTORC2 regulates hedgehog pathway activity by promoting stability to Gli2 protein and its nuclear translocation. Cell Death Dis. 2017;8:e2926 pubmed publisher
Yan B, Zhang Z, Jin D, Cai C, Jia C, Liu W, et al. mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation. Nat Commun. 2016;7:11151 pubmed publisher
Ji W, Yu Y, Li Z, Wang G, Li F, Xia W, et al. FGFR1 promotes the stem cell-like phenotype of FGFR1-amplified non-small cell lung cancer cells through the Hedgehog pathway. Oncotarget. 2016;7:15118-34 pubmed publisher
Vlčková K, Ondrušová L, Vachtenheim J, Réda J, Dundr P, Zadinová M, et al. Survivin, a novel target of the Hedgehog/GLI signaling pathway in human tumor cells. Cell Death Dis. 2016;7:e2048 pubmed publisher
Bora Singhal N, Perumal D, Nguyen J, Chellappan S. Gli1-Mediated Regulation of Sox2 Facilitates Self-Renewal of Stem-Like Cells and Confers Resistance to EGFR Inhibitors in Non-Small Cell Lung Cancer. Neoplasia. 2015;17:538-51 pubmed publisher
Larsen A, Bai R, Chung J, Borodovsky A, Rudin C, Riggins G, et al. Repurposing the antihelmintic mebendazole as a hedgehog inhibitor. Mol Cancer Ther. 2015;14:3-13 pubmed publisher
Amano K, Densmore M, Nishimura R, Lanske B. Indian hedgehog signaling regulates transcription and expression of collagen type X via Runx2/Smads interactions. J Biol Chem. 2014;289:24898-910 pubmed publisher
Kasaai B, Gaumond M, Moffatt P. Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation. J Biol Chem. 2013;288:13278-94 pubmed publisher
Roessler E, Ermilov A, Grange D, Wang A, Grachtchouk M, Dlugosz A, et al. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum Mol Genet. 2005;14:2181-8 pubmed
product information
Catalog Number :
17648
Product Name :
pCS2-MT GLI2 FL
article :
doi10.1093/hmg/ddi222
id2006
pubmed_id15994174
bacterial resistance :
Ampicillin
cloning :
backbonepCS2-MT
backbone_mutation
backbone_origin
backbone_size4350
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
There is an R250C mutation in the gene.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoterCMV IE94
sequencing_primer_3EBV-rev
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCJS, HPE9, PHS2, THP1, THP2
geneGLI2
id2736
genbank_ids
mutation
nameGLI2
shRNA_sequence
size4760
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6x Myc
resistance markers :
420
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA