This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-mDrc3-PA
catalog :
176467
citations: 1
Reference
Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, et al. Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 2020;16:e1008585 pubmed publisher
product information
Catalog Number :
176467
Product Name :
pCAG-mDrc3-PA
article :
doi10.1371/journal.pgen.1008585
id28212892
pubmed_id31961863
bacterial resistance :
Ampicillin
cloning :
backbonepCAG1.1
backbone_mutationpCAGGS was used as an original vector. PA sequence was added.
backbone_origin
backbone_size5211
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression vector of mouse dynein regulatory complex subunit 3 (Drc3) tagged with PA at C-terminus.
origin :
37
pi :
alt_names
Drc3
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3GCCACACCAGCCACCACCTTCTG
sequencing_primer_5GCCTTCTTCTTTTTCCTACAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases4930449E07Rik, Lrr, Lrrc48, m6Be, m6Bei
geneDrc3
id74665
genbank_ids
mutation
namedynein regulatory complex subunit 3
shRNA_sequence
size1587
species
10090
Mus musculus
tags
locationC terminal on backbone
tagPA
resistance markers :
1801
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA