pmr101A-hVPS26 | Addgene 17636 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pmr101A-hVPS26

catalog :

17636

citations: 3

Reference
Courtellemont T, De Leo M, Gopaldass N, Mayer A. CROP: a retromer-PROPPIN complex mediating membrane fission in the endo-lysosomal system. EMBO J. 2022;41:e109646 pubmed publisher
Li B, Wong C, Gao S, Zhang R, Sun R, Li Y, et al. The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking. elife. 2018;7: pubmed publisher
Shi H, Rojas R, Bonifacino J, Hurley J. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Nat Struct Mol Biol. 2006;13:540-8 pubmed

product information

Catalog Number :

17636

Product Name :

pmr101A-hVPS26

article :

doi	10.1038/nsmb1103
id	1995
pubmed_id	16732284

bacterial resistance :

Kanamycin

cloning :

backbone

modified pmr101

backbone_mutation

backbone_origin

ATCC

backbone_size

4100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

PCR was used to amplify the cDNA coding for human Vps26A and to add a C-terminal His5 tag (GLVPRGSHHHHH). The expressed recombinant protein contains the additional dipeptide MetGly at the N terminus as a result of the vector construction. Note: the publication lists NdeI as the 5' prime cloning site; however, this site is not unique.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NcoI? (see comments)
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HB58, Hbeta58, PEP8A, VPS26
gene	VPS26A
id	9559

genbank_ids

mutation

name

VPS26

shRNA_sequence

size

species

9606

Homo sapiens