This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ESCRT-II
catalog :
17633
citations: 3
Reference
Booth A, Marklew C, Ciani B, Beales P. The influence of phosphatidylserine localisation and lipid phase on membrane remodelling by the ESCRT-II/ESCRT-III complex. Faraday Discuss. 2021;232:188-202 pubmed publisher
Chiaruttini N, Redondo Morata L, Colom A, Humbert F, Lenz M, Scheuring S, et al. Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation. Cell. 2015;163:866-79 pubmed publisher
Hierro A, Sun J, Rusnak A, Kim J, Prag G, Emr S, et al. Structure of the ESCRT-II endosomal trafficking complex. Nature. 2004;431:221-5 pubmed
product information
Catalog Number :
17633
Product Name :
ESCRT-II
article :
doi10.1038/nature02914
id1992
pubmed_id15329733
bacterial resistance :
Ampicillin
cloning :
backbonepST39
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
DNAs for Vps25, Vps22, and Vps36 were cloned in order into pST39. Vps22 was tagged with an N-terminal hexahistidine tag and a TEV protease cleavage site; the other subunits were untagged.
origin :
37
pi :
alt_names
Vps25
Vps22
Vps36
cloning
clone_methodRestriction Enzyme
cloning_site_3See comments
cloning_site_5See comments
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGRD12, VAC3, VPL11
geneVPS36
id851135
aliasesVPL14, VPS22
geneSNF8
id856105
aliasesVPL12, VPT25
geneVPS25
id853566
genbank_ids
mutation
namepST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567)
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
216
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA