This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGST1-Osh4 (2-434)
catalog :
17632
citations: 2
Reference
Koag M, Cheun Y, Kou Y, Ouzon Shubeita H, Min K, Monzingo A, et al. Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4. Steroids. 2013;78:938-44 pubmed publisher
Im Y, Raychaudhuri S, Prinz W, Hurley J. Structural mechanism for sterol sensing and transport by OSBP-related proteins. Nature. 2005;437:154-8 pubmed
product information
Catalog Number :
17632
Product Name :
pGST1-Osh4 (2-434)
article :
doi10.1038/nature03923
id1991
pubmed_id16136145
bacterial resistance :
Ampicillin
cloning :
backbonemodified pGEX-4T
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
DNA encoding Osh4 residues 2 434 was amplified by PCR and subcloned into the BamHI and XhoI sites of a pGEX-4T vector modified to contain a TEV protease recognition site.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pGEX5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL145C, BSR3, LPI3, OSH4
geneKES1
id855958
genbank_ids
mutation
nameOsh4
shRNA_sequence
size1300
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagGST
resistance markers :
216
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA