EF.hICN1.Ubc.GFP | Addgene 17626 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

EF.hICN1.Ubc.GFP

catalog :

17626

citations: 7

Reference
Högstrand K, Darmanin S, Forshell T, Grandien A. Transformation of mouse T cells requires MYC and AKT activity in conjunction with inhibition of intrinsic apoptosis. Oncotarget. 2018;9:21396-21410 pubmed publisher
Brosh R, Hrynyk I, Shen J, Waghray A, Zheng N, Lemischka I. A dual molecular analogue tuner for dissecting protein function in mammalian cells. Nat Commun. 2016;7:11742 pubmed publisher
Bhola N, Jansen V, Koch J, Li H, Formisano L, Williams J, et al. Treatment of Triple-Negative Breast Cancer with TORC1/2 Inhibitors Sustains a Drug-Resistant and Notch-Dependent Cancer Stem Cell Population. Cancer Res. 2016;76:440-52 pubmed publisher
Gomi K, Arbelaez V, Crystal R, Walters M. Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway. PLoS ONE. 2015;10:e0116507 pubmed publisher
Forloni M, Dogra S, Dong Y, Conte D, Ou J, Zhu L, et al. miR-146a promotes the initiation and progression of melanoma by activating Notch signaling. elife. 2014;3:e01460 pubmed publisher
Séveno C, Loussouarn D, Bréchet S, Campone M, Juin P, Barillé Nion S. ?-Secretase inhibition promotes cell death, Noxa upregulation, and sensitization to BH3 mimetic ABT-737 in human breast cancer cells. Breast Cancer Res. 2012;14:R96 pubmed
Yu X, Alder J, Chun J, Friedman A, Heimfeld S, Cheng L, et al. HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Stem Cells. 2006;24:876-88 pubmed

product information

Catalog Number :

17626

Product Name :

EF.hICN1.Ubc.GFP

article :

doi	10.1634/stemcells.2005-0598
id	1988
pubmed_id	16513761

bacterial resistance :

Ampicillin

cloning :

backbone

cFUGW

backbone_mutation

backbone_origin

backbone_size

11500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function. hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

origin :

pi :

alt_names

intracellular domain of the Notch receptor

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	EF-1a Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AOS5, AOVD1, TAN1, hN1
gene	NOTCH1
id	4851

genbank_ids

mutation

name

ICN1

shRNA_sequence

size

2400

species

9606

Homo sapiens