EF.hHES1.Ubc.GFP | Addgene 17624 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

EF.hHES1.Ubc.GFP

catalog :

17624

citations: 5

Reference
Fehrenbach S, Novak D, Bernhardt M, Larribere L, Boukamp P, Umansky V, et al. Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage. Sci Rep. 2016;6:28891 pubmed publisher
Yuan R, Ke J, Sun L, He Z, Zou Y, He X, et al. HES1 promotes metastasis and predicts poor survival in patients with colorectal cancer. Clin Exp Metastasis. 2015;32:169-79 pubmed publisher
So J, Wahler J, Das Gupta S, Salerno D, Maehr H, Uskokovic M, et al. HES1-mediated inhibition of Notch1 signaling by a Gemini vitamin D analog leads to decreased CD44(+)/CD24(-/low) tumor-initiating subpopulation in basal-like breast cancer. J Steroid Biochem Mol Biol. 2015;148:111-21 pubmed publisher
Kwon O, Valdez J, Zhang L, Zhang B, Wei X, Su Q, et al. Increased Notch signalling inhibits anoikis and stimulates proliferation of prostate luminal epithelial cells. Nat Commun. 2014;5:4416 pubmed publisher
Yu X, Alder J, Chun J, Friedman A, Heimfeld S, Cheng L, et al. HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Stem Cells. 2006;24:876-88 pubmed

product information

Catalog Number :

17624

Product Name :

EF.hHES1.Ubc.GFP

article :

doi	10.1634/stemcells.2005-0598
id	1988
pubmed_id	16513761

bacterial resistance :

Ampicillin

cloning :

backbone

cFUGW

backbone_mutation

backbone_origin

backbone_size

11500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

hHES1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

origin :

pi :

alt_names

hHES1

Hes-1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	EF-1a Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HES-1, HHL, HRY, bHLHb39
gene	HES1
id	3280

genbank_ids

mutation

name

HES1

shRNA_sequence

size

850

species

9606

Homo sapiens