EF.hICN1.CMV.GFP | Addgene 17623 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

EF.hICN1.CMV.GFP

catalog :

17623

citations: 13

Reference
Lim J, Straub E, Stubenrauch F, Iftner T, Schindler M, Simon C. An enhanced triple fluorescence flow-cytometry-based assay shows differential activation of the Notch signaling pathway by human papillomavirus E6 proteins. Sci Rep. 2022;12:3000 pubmed publisher
Miao K, Lei J, Valecha M, Zhang A, Xu J, Wang L, et al. NOTCH1 activation compensates BRCA1 deficiency and promotes triple-negative breast cancer formation. Nat Commun. 2020;11:3256 pubmed publisher
Wang F, Hou W, Chitsike L, Xu Y, Bettler C, Perera A, et al. ABL1, Overexpressed in Hepatocellular Carcinomas, Regulates Expression of NOTCH1 and Promotes Development of Liver Tumors in Mice. Gastroenterology. 2020;159:289-305.e16 pubmed publisher
Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. PLoS ONE. 2019;14:e0226570 pubmed publisher
Ganguly S, Hostetter G, Tang L, Frank S, Saboda K, Mehra R, et al. Notch3 promotes prostate cancer-induced bone lesion development via MMP-3. Oncogene. 2020;39:204-218 pubmed publisher
Kahn S, Wang X, Nitta R, Gholamin S, Theruvath J, Hutter G, et al. Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma. Nat Commun. 2018;9:4121 pubmed publisher
Ramakrishnan S, Hu Q, Krishnan N, Wang D, Smit E, Granger V, et al. Decitabine, a DNA-demethylating agent, promotes differentiation via NOTCH1 signaling and alters immune-related pathways in muscle-invasive bladder cancer. Cell Death Dis. 2017;8:3217 pubmed publisher
Huang C, Iwagami Y, Aihara A, Chung W, de la Monte S, Thomas J, et al. Anti-Tumor Effects of Second Generation Î²-Hydroxylase Inhibitors on Cholangiocarcinoma Development and Progression. PLoS ONE. 2016;11:e0150336 pubmed publisher
De Ford C, Heidersdorf B, Haun F, Murillo R, Friedrich T, Borner C, et al. The clerodane diterpene casearin J induces apoptosis of T-ALL cells through SERCA inhibition, oxidative stress, and interference with Notch1 signaling. Cell Death Dis. 2016;7:e2070 pubmed publisher
Lee S, Lee S, Yang H, Song S, Kim K, Saunders T, et al. Notch pathway targets proangiogenic regulator Sox17 to restrict angiogenesis. Circ Res. 2014;115:215-26 pubmed publisher
Liu Y, Yang C, Huang M, Yeh C, Wu A, Lee Y, et al. Cisplatin selects for multidrug-resistant CD133+ cells in lung adenocarcinoma by activating Notch signaling. Cancer Res. 2013;73:406-16 pubmed publisher
Wu N, Rollin J, Masse I, Lamartine J, Gidrol X. p63 regulates human keratinocyte proliferation via MYC-regulated gene network and differentiation commitment through cell adhesion-related gene network. J Biol Chem. 2012;287:5627-38 pubmed publisher
Yu X, Alder J, Chun J, Friedman A, Heimfeld S, Cheng L, et al. HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Stem Cells. 2006;24:876-88 pubmed

product information

Catalog Number :

17623

Product Name :

EF.hICN1.CMV.GFP

article :

doi	10.1634/stemcells.2005-0598
id	1988
pubmed_id	16513761

bacterial resistance :

Ampicillin

cloning :

backbone

EF.v-CMV.GFP

backbone_mutation

backbone_origin

Linzhao Cheng Lab

backbone_size

8200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function. The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

origin :

pi :

alt_names

intracellular domain of the Notch receptor

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	EF-1a Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AOS5, AOVD1, TAN1, hN1
gene	NOTCH1
id	4851

genbank_ids

mutation

name

ICN1

shRNA_sequence

size

2400

species

9606

Homo sapiens