This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DR.GFP
catalog :
17617
citations: 2
| Reference |
|---|
Cui Y, Golob J, Kelleher E, Ye Z, Pardoll D, Cheng L. Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells. Blood. 2002;99:399-408 pubmed
|
product information
Catalog Number :
17617
Product Name :
DR.GFP
article :
| doi | 10.1182/blood.V99.2.399 |
| id | 1984 |
| pubmed_id | 11781219 |
bacterial resistance :
Ampicillin
cloning :
| backbone | SIN LV pRLLhPGK.GFP Sin-18 (modified) | ||
| backbone_mutation | |||
| backbone_origin | Dr. Didier Trono | ||
| backbone_size | |||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs). We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells. Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 g for 10^6 293T cells.
origin :
37
pi :
|
plasmid copy :
SIN LV pRLLhPGK.GFP Sin-18 (abbreviated as PGK.GFP) was kindly provided by Dr. Didier Trono.
resistance markers :
| 76 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments