This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBPGw
catalog :
17574
citations: 6
Reference
Xu W, Rustenhoven J, Nelson C, Dykstra T, Ferreiro A, Papadopoulos Z, et al. A novel immune modulator IM33 mediates a glia-gut-neuronal axis that controls lifespan. Neuron. 2023;111:3244-3254.e8 pubmed publisher
Bedont J, Toda H, Shi M, Park C, Quake C, Stein C, et al. Short and long sleeping mutants reveal links between sleep and macroautophagy. elife. 2021;10: pubmed publisher
Ahmed O, Avila Herrera A, Tun K, Serpa P, Peng J, Parthasarathy S, et al. Evolution of Mechanisms that Control Mating in Drosophila Males. Cell Rep. 2019;27:2527-2536.e4 pubmed publisher
Lin S, Dikler S, Blincoe W, Ferguson R, Sheridan R, Peng Z, et al. Mapping the dark space of chemical reactions with extended nanomole synthesis and MALDI-TOF MS. Science. 2018;361: pubmed publisher
Janssens D, Hamm D, Anhezini L, Xiao Q, Siller K, Siegrist S, et al. An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and Rapid Restriction of Developmental Potential during Asymmetric Stem Cell Division. Dev Cell. 2017;40:367-380.e7 pubmed publisher
Pfeiffer B, Jenett A, Hammonds A, Ngo T, Misra S, Murphy C, et al. Tools for neuroanatomy and neurogenetics in Drosophila. Proc Natl Acad Sci U S A. 2008;105:9715-20 pubmed publisher
product information
Catalog Number :
17574
Product Name :
pBPGw
article :
doi10.1073/pnas.0803697105
id1972
pubmed_id18621688
bacterial resistance :
Chloramphenicol and Ampicillin
cloning :
backbonepBDP
backbone_mutation
backbone_origin
backbone_size8902
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
pBPGw is a modular Gateway compatible promoter-less GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest.
growth temp :
Bacterial Strain DB3.1 (Invitrogen) or similar must be used to propagate plasmids carrying the ccdB gene.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoter
sequencing_primer_3BDP R (bp 5,286) : ATAATGGTGCAGGGCGCTGAC
sequencing_primer_5BDP F (bp 11,503): AAATAGGGGTTCCGCGCACAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL248C, GAL81
geneGAL4
id855828
genbank_ids
mutation
nameGAL4
shRNA_sequence
size2646
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
GAL4 leader, CDS, and hsp70 polyA were cloned from pGaTB (via DGRC).
resistance markers :
428
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA