This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+)_Pmel-1_TCRB
catalog :
175288
product information
Catalog Number :
175288
Product Name :
pcDNA3.1(+)_Pmel-1_TCRB
article :
doi10.1101/2021.11.30.470597
id28207612
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originGenscript
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Suitable for IVT
growth notes :
Overwijk, Willem W., Marc R. Theoret, Steven E. Finkelstein, Deborah R. Surman, Laurina A. de Jong, Florry A. Vyth-Dreese, Trees A. Dellemijn, et al. 2003. Tumor Regression and Autoimmunity after Reversal of a Functionally Tolerant State of Self-Reactive CD8+ T Cells. The Journal of Experimental Medicine 198 (4): 569 80.
growth strain :
Expresses Pmel-1 TCR beta chain
origin :
37
pi :
alt_names
T cell receptor beta chain mRNA, complete cds
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5AflII
promoterCMV and T7
sequencing_primer_3BGH-Rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTCRbeta, Tib
geneTcrb
id21577
genbank_ids
EF154514.1
mutation
nameT cell receptor beta chain
shRNA_sequence
size954
species
10090
Mus musculus
tags
plasmid copy :
The vector was from Genscript. The insert sequence was taken from GenBank (EF154514.1). Reference cited below in comments:
resistance markers :
4584
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA