pAAV-SynaptoTAG2 | Addgene 175275 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-SynaptoTAG2

catalog :

175275

citations: 1

Reference
Li E, Guo J, Oh S, Luo Y, Oliveros H, Du W, et al. Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D. Nat Methods. 2021;18:1542-1551 pubmed publisher

product information

Catalog Number :

175275

Product Name :

pAAV-SynaptoTAG2

article :

doi	10.1038/s41592-021-01319-9
id	28220252
pubmed_id	34824475

bacterial resistance :

Ampicillin

cloning :

backbone

AAV2

backbone_mutation

backbone_origin

backbone_size

4561

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

AAV

growth notes :

Sequencing may be difficult due to secondary structure/dimerization. The following primers have been used in the Xu lab: 5': actcagcgctgcctcagtc (upstream of tdTomato) 3': ggccatgttgttgtcctcggaggaggcggtgc (in tdTomato) 5': gggcatggcaccggcagcaccggcagcggcagc (in tdTomato) 3': gctgaacttgtggccgtttacgtcg (in EGFP) 3': cagggtcagcttgccgtaggtggcatcg (in EGFP)

growth strain :

AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	Synapsin
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RATVAMPB, RATVAMPIR, SYB, Syb2
gene	Vamp2
id	24803

genbank_ids

mutation

name

EGFP-Synaptobrevin-2; tdTomato

shRNA_sequence

size

2576

species

32644

Other