This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCherry-FKBP-E2
catalog :
175243
citations: 1
Reference
Peeples W, Rosen M. Mechanistic dissection of increased enzymatic rate in a phase-separated compartment. Nat Chem Biol. 2021;17:693-702 pubmed publisher
product information
Catalog Number :
175243
Product Name :
mCherry-FKBP-E2
article :
doi10.1038/s41589-021-00801-x
id28220236
pubmed_id34035521
bacterial resistance :
Ampicillin
cloning :
backbonepMagic
backbone_mutation
backbone_originlab stock, see comments
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3CAAGGGGTTATGCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC358B7.1, P18, UBC9
geneUBE2I
id7329
genbank_ids
mutation
nameUBE2I
shRNA_sequence
size1623
species
9606
Homo sapiens
tags
resistance markers :
4315
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA