This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDD2603
catalog :
175087
citations: 1
Reference
Alzhanova D, Meyo J, Juarez A, Dittmer D. The ORF45 Protein of Kaposi Sarcoma-Associated Herpesvirus Is an Inhibitor of p53 Signaling during Viral Reactivation. J Virol. 2021;95:e0145921 pubmed publisher
product information
Catalog Number :
175087
Product Name :
pDD2603
article :
doi10.1128/JVI.01459-21
id28220222
pubmed_id34523970
bacterial resistance :
Ampicillin
cloning :
backbonepEF1/Myc-His_A
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Used genscript site-directed mutagenesis
growth strain :
Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
origin :
37
pi :
alt_names
195
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHV8GK18_gp52
geneORF50
id4961526
genbank_ids
mutationT504A, T508A
nameKSHV RTA phospho-mutant (ORF50)
shRNA_sequence
size
species
32644
Other
Kaposi's sarcoma-associated herpesvirus 8
tags
plasmid copy :
WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378)
resistance markers :
1919
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA