This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDD2711
catalog :
175083
citations: 1
Reference
Alzhanova D, Meyo J, Juarez A, Dittmer D. The ORF45 Protein of Kaposi Sarcoma-Associated Herpesvirus Is an Inhibitor of p53 Signaling during Viral Reactivation. J Virol. 2021;95:e0145921 pubmed publisher
product information
Catalog Number :
175083
Product Name :
pDD2711
article :
doi10.1128/JVI.01459-21
id28220222
pubmed_id34523970
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
KSHV ORF45 carrying E223A-S226A (Alzhanova, 2021)/RC (Li, 2009) mutations and C-terminal Flag was synthesized and cloned into pcDNA3.1(+) using EcoRI and XhoI sites
growth strain :
Mammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutations
origin :
37
pi :
alt_names
80
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHV8GK18_gp51
geneORF45
id4961474
genbank_ids
mutationE223A, S226A, RC mutations
nameKSHV ORF45
shRNA_sequence
size
species
32644
Other
Kaposi's sarcoma-associated herpesvirus 8
tags
plasmid copy :
Dr. Fanxiu Zhu (Florida State University), PMID: 19116250.
resistance markers :
1919
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA