This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pVC-FUS-R521C
catalog :
175067
citations: 1
Reference
Wan Q, Mouton S, Veenhoff L, Boersma A. A FRET-based method for monitoring structural transitions in protein self-organization. Cell Rep Methods. 2022;2:100184 pubmed publisher
product information
Catalog Number :
175067
Product Name :
pVC-FUS-R521C
article :
doi10.1016/j.crmeth.2022.100184
id28220106
pubmed_id35475219
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutation
backbone_originPromega
backbone_size5526
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells
origin :
37
pi :
alt_names
FUS(R521C)
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5NheI
promoterCMV Promoter
sequencing_primer_3AAAACCTCCCACATCTCCCC
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS
geneFUS
id2521
genbank_ids
CAA50559.1
mutationchanged Arginin 521 to Cystein; mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK); mCherry: C-Terminal deletion of 5 aa (DELYK) and N-Terminal deletion of 7 aa (MVSKGEE)
nameFUS glycine rich protein
shRNA_sequence
size2952
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmVenus + mCherry
locationN terminal on insert
tagHA
resistance markers :
5565
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA