This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
eSpCas9-EF-5Flnc
catalog :
174870
citations: 1
Reference
Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, et al. STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity. Nat Commun. 2022;13:7672 pubmed publisher
product information
Catalog Number :
174870
Product Name :
eSpCas9-EF-5Flnc
article :
doi10.1038/s41467-022-35286-2
id28220199
pubmed_id36539402
bacterial resistance :
Ampicillin
cloning :
backboneeSpCas9(1.1)
backbone_mutationWe applied an optimized sgRNA design (sgRNA(F+E)) (Chen et al., Cell, 2013).
backbone_originFeng Zhang
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth strain :
CRISPR vector for generating Flnc STREAMING-tag KI cell
origin :
37
pi :
alt_names
Flnc (Mus musculus)
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3
sequencing_primer_5LKO1_5_primer
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases1110055E19Rik, ABP-280, ABPL, Fl, Fln2
geneFlnc
id68794
genbank_ids
mutation
namesgRNA for mouse Flnc
shRNA_sequenceTCCAGCAGAACACATTCACG
size
species
100
Synthetic
tags
resistance markers :
2244
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA