This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-MmPeg10rc4
catalog :
174858
citations: 2
Reference
Ohtsuka M, Imafuku J, Hori S, Kurosaki A, Nakamura A, Nakahara T, et al. Delivering mRNAs to mouse tissues using the SEND system. bioRxiv. 2023;: pubmed publisher
SEGEL M, Lash B, Song J, Ladha A, Liu C, Jin X, et al. Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery. Science. 2021;373:882-889 pubmed publisher
product information
Catalog Number :
174858
Product Name :
pCMV-MmPeg10rc4
article :
doi10.1126/science.abg6155
id28220197
pubmed_id34413232
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that the Addgene verified sequence is slightly different from the sequence reported in the publication. The plasmid works as described and an update map is included in the supplemental documents section above.
growth strain :
Expresses recoded mouse Peg10 in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAA407948, Edr, HB-1, MEF3L, Mar2, Mart2, MyEF-3, Rtl2
genePeg10
id170676
genbank_ids
NM_130877.2
mutation
namerecoded Mus musculus PEG10
shRNA_sequence
size2897
species
10090
Mus musculus
tags
locationC terminal on insert
tagHA
resistance markers :
898
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA