pET28_6xHis-PARP1-A774L | Addgene 174796 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET28_6xHis-PARP1-A774L

catalog :

174796

citations: 1

Reference
Ooi S, Sato S, Tomomori Sato C, Zhang Y, Wen Z, Banks C, et al. Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher

product information

Catalog Number :

174796

Product Name :

pET28_6xHis-PARP1-A774L

article :

doi	10.1073/pnas.2107277118
id	28220185
pubmed_id	34465625

bacterial resistance :

Kanamycin

cloning :

backbone

pET28

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Protein residues 1-1014 (V762A, A774L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation)

growth strain :

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1
gene	PARP1
id	142

genbank_ids

mutation

A774L, V762A

name

PARP1-A774L

shRNA_sequence

size

species

9606

Homo sapiens