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product summary
company name :
Addgene
product type :
cDNA
product name :
pKan cpRBP-FL P53
catalog :
174504
citations: 1
Reference
Ha J, Yu X, Carpizo D, Loh S. Urea Denaturation, Zinc Binding, and DNA Binding Assays of Mutant p53 DNA-binding Domains and Full-length Proteins. Bio Protoc. 2021;11:e4188 pubmed publisher
product information
Catalog Number :
174504
Product Name :
pKan cpRBP-FL P53
article :
doi10.21769/BioProtoc.4188
id28220123
pubmed_id34786438
bacterial resistance :
Kanamycin
cloning :
backbonepET41
backbone_mutationThe synthetic gene of Thermoanaerobacter tencongenesis ribose binding protein (tteRBP) circular permutant with N-terminal HisTag is inserted between NdeI and XhoI sites. The original N- and C-termini of tteRBP are linked with 58 amino acid linker containing two HRV3C protease sites.
backbone_originNovagen
backbone_size5933
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
origin :
37
pi :
alt_names
TP53
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NotI
promoterT7
sequencing_primer_3GGTACTGATGACCAGACCG
sequencing_primer_5CCCTAATTTTATCCCCGCTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
nameP53 gene
shRNA_sequence
size1185
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagtteRBP circular permutant (97 - 277 amino acids) with N-terminal histag and 27amino acid linker containing HRV3C protease site
locationC terminal on backbone
tag20 amino acid linker containing HRV3C protease site followed by tteRBP circular permutant (1 -96 amino acid)
plasmid copy :
The original P53 gene sequence was obtained the plasmid, PHP53B (purchased from ATCC).
resistance markers :
5629
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA