pnEA-vH_His-TEV-DSep1 | Addgene 174493 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pnEA-vH_His-TEV-DSep1

catalog :

174493

citations: 1

Reference
Mavrakis M, Azou Gros Y, Tsai F, Alvarado J, Bertin A, Iv F, et al. Septins promote F-actin ring formation by crosslinking actin filaments into curved bundles. Nat Cell Biol. 2014;16:322-34 pubmed publisher

product information

Catalog Number :

174493

Product Name :

pnEA-vH_His-TEV-DSep1

article :

doi	10.1038/ncb2921
id	28223036
pubmed_id	24633326

bacterial resistance :

Ampicillin

cloning :

backbone

pnEA-vH

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Plasmid used in the following publications: Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms. Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484. Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly. M Mavrakis, F-C Tsai, G H Koenderink Methods Cell Biol 2016;136:199-220. doi: 10.1016/bs.mcb.2016.03.020. Epub 2016 Jun 14. PMID: 27473911 DOI: 10.1016/bs.mcb.2016.03.020

growth strain :

bacterial expression of Drosophila Sep1

origin :

pi :

alt_names

Septin 1

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7 terminal
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG1403, 38960, CG1403, DSep1, Diff6, Dmel CG1403, anon-19Fa, dSEPT1, iby, sep1, tu1
gene	Sep1
id	33114

genbank_ids

mutation

name

Sep1

shRNA_sequence

size

species

7227

Drosophila melanogaster