This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2-Jmjd3-F
catalog :
17440
citations: 8
Reference
Rana S, Maurya S, Mohapatra G, Singh S, Babar R, Chandrasekhar H, et al. Activation of epigenetic regulator KDM6B by Salmonella Typhimurium enables chronic infections. Gut Microbes. 2021;13:1986665 pubmed publisher
Gao L, Yang M, Wang X, Yang L, Bai C, Li G. Mstn knockdown decreases the trans-differentiation from myocytes to adipocytes by reducing Jmjd3 expression via the SMAD2/SMAD3 complex. Biosci Biotechnol Biochem. 2019;83:2090-2096 pubmed publisher
Song A, Jiang S, Wang Q, Zou J, Lin Z, Gao X. JMJD3 Is Crucial for the Female AVPV RIP-Cre Neuron-Controlled Kisspeptin-Estrogen Feedback Loop and Reproductive Function. Endocrinology. 2017;158:1798-1811 pubmed publisher
Ueno K, Iwagawa T, Kuribayashi H, Baba Y, Nakauchi H, Murakami A, et al. Transition of differential histone H3 methylation in photoreceptors and other retinal cells during retinal differentiation. Sci Rep. 2016;6:29264 pubmed publisher
Wang Y, Li Y, Guo C, Lu Q, Wang W, Jia Z, et al. ISL1 and JMJD3 synergistically control cardiac differentiation of embryonic stem cells. Nucleic Acids Res. 2016;44:6741-55 pubmed publisher
Kamikawa Y, Donohoe M. The localization of histone H3K27me3 demethylase Jmjd3 is dynamically regulated. Epigenetics. 2014;9:834-41 pubmed publisher
Wang W, Tzeng T, Wang J, Lee D, Lin Y, Wu P, et al. The EP300, KDM5A, KDM6A and KDM6B chromatin regulators cooperate with KLF4 in the transcriptional activation of POU5F1. PLoS ONE. 2012;7:e52556 pubmed publisher
Hong S, Cho Y, Yu L, Yu H, Veenstra T, Ge K. Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Proc Natl Acad Sci U S A. 2007;104:18439-44 pubmed
product information
Catalog Number :
17440
Product Name :
pCS2-Jmjd3-F
article :
doi10.1073/pnas.0707292104
id1949
pubmed_id18003914
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+ NLSMT
backbone_mutation
backbone_origin
backbone_size4400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
growth strain :
Expression of Jmjd3 in mammalian cells
origin :
37
pi :
alt_names
jumonji domain containing 3
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5XhoI
promoter
sequencing_primer_3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliases1700064E03Rik, Jmjd3
geneKdm6b
id216850
genbank_ids
BC075632
mutation
nameJMJD3
shRNA_sequence
size5000
species
10090
Mus musculus
tags
locationN terminal on backbone
tag6xMyc
locationC terminal on insert
tagFlag
resistance markers :
159
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA