pCS2-UTY-F | Addgene 17439 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCS2-UTY-F

catalog :

17439

citations: 5

Reference
Schüchner S, Behm C, Mudrak I, Ogris E. The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context. Sci Signal. 2020;13: pubmed publisher
Gozdecka M, Meduri E, Mazan M, Tzelepis K, Dudek M, Knights A, et al. UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs. Nat Genet. 2018;50:883-894 pubmed publisher
Walport L, Hopkinson R, Vollmar M, Madden S, Gileadi C, Oppermann U, et al. Human UTY(KDM6C) is a male-specific NÏµ-methyl lysyl demethylase. J Biol Chem. 2014;289:18302-13 pubmed publisher
Shpargel K, Sengoku T, Yokoyama S, Magnuson T. UTX and UTY demonstrate histone demethylase-independent function in mouse embryonic development. PLoS Genet. 2012;8:e1002964 pubmed publisher
Hong S, Cho Y, Yu L, Yu H, Veenstra T, Ge K. Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Proc Natl Acad Sci U S A. 2007;104:18439-44 pubmed

product information

Catalog Number :

17439

Product Name :

pCS2-UTY-F

article :

doi	10.1073/pnas.0707292104
id	1949
pubmed_id	18003914

bacterial resistance :

Ampicillin

cloning :

backbone

pCS2+ NLSMT

backbone_mutation

backbone_origin

backbone_size

4400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Full length human UTY with a C-terminal FLAG was PCR-amplified from human monocytic leukemia cell line THP-1 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.

growth strain :

Expression of UTY in mammalian cells

origin :

pi :

alt_names

ubiquitously transcribed tetratricopeptide repeat gene, Y-linked

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	XhoI
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	UTY1
gene	UTY
id	7404

genbank_ids

NM_007125

mutation

name

UTY

shRNA_sequence

size

4000

species

9606

Homo sapiens