This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p406MET3
catalog :
17436
product information
Catalog Number :
17436
Product Name :
p406MET3
article :
doi
id1947
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonep406MET3-cyc1t
backbone_mutation
backbone_originA. Geoghegan (Rockefeller University)
backbone_size4844
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
origin :
37
pi :
alt_names
ATP sulfurylase
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5SalI
promoter
sequencing_primer_3
sequencing_primer_5M13 Reverse
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneMET3
id853466
genbank_ids
mutationMET3 promoter is between SacI and XbaI restriction sites.
nameMET3 promoter + polylinker + CYC1 terminator
shRNA_sequence
size260
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
Original vector p406MET3-cyc1t was created by subcloning PCR fragment of genomic MET3 promoter (with SacI and XbaI restriction sites introduced) into SacI-XbaI cut vector pRS406. This plasmid does not contain CYC1 terminator. Plasmid p406MET3 was created by subcloning SalI-KpnI insert from p405MET25 (insert = CYC1 terminator) into SalI-KpnI cut p406MET3-cyc1t vector.
resistance markers :
55
99
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA