This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC1-ORAI1-EC-HA
catalog :
174335
citations: 1
Reference
Wu B, Woo J, Vila P, Jew M, Leung J, Sun Z, et al. NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca2+ entry in T cells. Cell Rep. 2021;36:109603 pubmed publisher
product information
Catalog Number :
174335
Product Name :
pEGFPC1-ORAI1-EC-HA
article :
doi10.1016/j.celrep.2021.109603
id28220097
pubmed_id34433025
bacterial resistance :
Kanamycin
cloning :
backbonepEGFPN1
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5XhoI
promoterCMV
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCRACM1, IMD9, ORAT1, TAM2, TMEM142A
geneORAI1
id84876
genbank_ids
NM_032790.3
NM_032790.3
mutation
namehuman Orai1
shRNA_sequenceG ATC GGC CAG AGT TAC TCC G
size1000
species
9606
Homo sapiens
tags
location
tagGFP in the N-terminus and HA tag in the extracellular loop 2 of ORAI1
resistance markers :
5674
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA