This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Puro.Cre empty vector
catalog :
17408
citations: 14
Reference
Lapierre M, Lawler K, Godbersen S, Farooqi I, Stoffel M. MicroRNA-7 regulates melanocortin circuits involved in mammalian energy homeostasis. Nat Commun. 2022;13:5733 pubmed publisher
Tournaire G, Loopmans S, Stegen S, Rinaldi G, Eelen G, Torrekens S, et al. Skeletal progenitors preserve proliferation and self-renewal upon inhibition of mitochondrial respiration by rerouting the TCA cycle. Cell Rep. 2022;40:111105 pubmed publisher
Qiao F, Law H, Krieger K, Clement E, Xiao Y, Buckley S, et al. Ctdp1 deficiency leads to early embryonic lethality in mice and defects in cell cycle progression in MEFs. Biol Open. 2021;10: pubmed publisher
Alexander C, Barzik M, Fujiwara I, Remmert K, Wang Y, Petralia R, et al. Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation. FASEB J. 2021;35:e21092 pubmed publisher
Thomas P, Schafler E, Ruff S, Voisin M, Ha S, Logan S. UXT in Sertoli Cells is Required for Blood-Testis Barrier Integrity. Biol Reprod. 2020;: pubmed publisher
Su X, Jin Y, Shen Y, Kim I, Weintraub N, Tang Y. RNAase III-Type Enzyme Dicer Regulates Mitochondrial Fatty Acid Oxidative Metabolism in Cardiac Mesenchymal Stem Cells. Int J Mol Sci. 2019;20: pubmed publisher
Tao Y, Kang B, Petkovich D, Bhandari Y, In J, Stein O Brien G, et al. Aging-like Spontaneous Epigenetic Silencing Facilitates Wnt Activation, Stemness, and BrafV600E-Induced Tumorigenesis. Cancer Cell. 2019;35:315-328.e6 pubmed publisher
Berenguer J, Lagerweij T, Zhao X, Dusoswa S, van der Stoop P, Westerman B, et al. Glycosylated extracellular vesicles released by glioblastoma cells are decorated by CCL18 allowing for cellular uptake via chemokine receptor CCR8. J Extracell Vesicles. 2018;7:1446660 pubmed publisher
Nishimura M, Watanabe T, Yagi S, Yamanaka T, Fujimuro M. Kaposi's sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production. Sci Rep. 2017;7:329 pubmed publisher
Home P, Kumar R, Ganguly A, Saha B, Milano Foster J, Bhattacharya B, et al. Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development. Development. 2017;144:876-888 pubmed publisher
Zhang K, Guo Y, Wang X, Zhao H, Ji Z, Cheng C, et al. WNT/β-Catenin Directs Self-Renewal Symmetric Cell Division of hTERThigh Prostate Cancer Stem Cells. Cancer Res. 2017;77:2534-2547 pubmed publisher
de Jong P, Taniguchi K, Harris A, Bertin S, Takahashi N, Duong J, et al. ERK5 signalling rescues intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation. Nat Commun. 2016;7:11551 pubmed publisher
Beaulieu Y, Leon Machado J, Ethier S, Gaudreau L, Steimle V. Degradation, Promoter Recruitment and Transactivation Mediated by the Extreme N-Terminus of MHC Class II Transactivator CIITA Isoform III. PLoS ONE. 2016;11:e0148753 pubmed publisher
Kumar M, Erkeland S, Pester R, Chen C, Ebert M, Sharp P, et al. Suppression of non-small cell lung tumor development by the let-7 microRNA family. Proc Natl Acad Sci U S A. 2008;105:3903-8 pubmed publisher
product information
Catalog Number :
17408
Product Name :
Puro.Cre empty vector
article :
doi10.1073/pnas.0712321105
id2004
pubmed_id18308936
bacterial resistance :
Ampicillin
cloning :
backbonePuro.Cre
backbone_mutation
backbone_originJacks Lab
backbone_size9200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Cre/Lox
growth notes :
Puro.Cre was initially generated by amplification of PuroR from MSCV.Puro (Clontech) with PuroF (5 -CGCTGCGCCGAATTCATGACCGAGTACAAGCCCACG-3 ) and PuroR (5 -TAAGCGGCCGCTCGAGTCAGGCACCGGGCTTGCGGGTC-3 ), digestion with EcoRI and NotI and cloning into UbC.Luciferase.PGK.Cre to generate Puro.Cre. For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3MSCV rev
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name
shRNA_sequence
size
species
tags
plasmid copy :
UbC.Luciferase.PGK.Cre was a kind gift of M. Dupage
resistance markers :
225
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA