This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDF RBP-FL P53
catalog :
174042
citations: 1
Reference
Blanden A, Yu X, Blayney A, Demas C, Ha J, Liu Y, et al. Zinc shapes the folding landscape of p53 and establishes a pathway for reactivating structurally diverse cancer mutants. elife. 2020;9: pubmed publisher
product information
Catalog Number :
174042
Product Name :
pCDF RBP-FL P53
article :
doi10.7554/eLife.61487
id28220042
pubmed_id33263541
bacterial resistance :
Streptomycin
cloning :
backbonepCDFDuet-1
backbone_mutationtteRBP gene with 14 amino acid linker (GGAASGGAAGGSSAA) was inserted between SacI and AscI restriction enzyme sites.
backbone_originNovagen
backbone_size3781
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)
growth temp :
For protein expression, please use BL21(DE3).
origin :
37
pi :
alt_names
TP53
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoterT7
sequencing_primer_3T7T
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
nameP53 gene
shRNA_sequence
size1182
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagThermoanaerobacter Tencongenesis ribose binding protein with N-terminal histag
plasmid copy :
The original P53 gene sequence was obtained from the plasmid, PHP53B (purchased from ATCC).
resistance markers :
5629
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA