pcDNA HisB TO rFez1 | Addgene 17385 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA HisB TO rFez1

catalog :

17385

citations: 1

Reference
Naghavi M, Hatziioannou T, Gao G, Goff S. Overexpression of fasciculation and elongation protein zeta-1 (FEZ1) induces a post-entry block to retroviruses in cultured cells. Genes Dev. 2005;19:1105-15 pubmed

product information

Catalog Number :

17385

Product Name :

pcDNA HisB TO rFez1

article :

doi	10.1101/gad.1290005
id	1946
pubmed_id	15879557

bacterial resistance :

Ampicillin

cloning :

backbone

pCDNA4 TO2 myc HisB

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Full-length Rat2 FEZ1 sequences, 1179 bp, was amplified using cDNA from the wild-type Rat2 fibroblasts or the mutant R3-2 line as templates. The sequences of the PCR primers for generation of C-terminal myc-His-tagged FEZ1 were as follows: forward primer, FEZ1-S1-BamHI, 5'-GCTGATGGATCCACCATGGAGGCTC CACTGGTGAGT3'; reverse primer, FEZ1-A3-XhoI, 5'-GCAACGCTCGAGCAGGTTGGACAGAGCACTTTTAAA-3'.

origin :

pi :

alt_names

Fez1

fasciculation and elongation protein zeta-1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Fez1
id	81730

genbank_ids

mutation

name

FEZ1

shRNA_sequence

size

1200

species

10116

Rattus norvegicus