pcDNA4-TO-Myc-rZAP | Addgene 17381 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA4-TO-Myc-rZAP

catalog :

17381

citations: 2

Reference
Moldovan J, Moran J. The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition. PLoS Genet. 2015;11:e1005121 pubmed publisher
Gao G, Guo X, Goff S. Inhibition of retroviral RNA production by ZAP, a CCCH-type zinc finger protein. Science. 2002;297:1703-6 pubmed

product information

Catalog Number :

17381

Product Name :

pcDNA4-TO-Myc-rZAP

article :

doi	10.1126/science.1074276
id	1945
pubmed_id	12215647

bacterial resistance :

Ampicillin

cloning :

backbone

pCDNA4 TO2 myc HisB

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The NZAP fragment was excised from pBabe-NZAP-Zeo with EcoRI-NotI and cloned into pCDNA4/TO2-myc-HisB to generate a myc-tagged NZAP. The C-terminal portion of ZAP was cloned from a Rat2 cell cDNA library by PCR using sense primer CZAP-SP (5 -GAGCTCTCTGGGCTTAACC-3 ) and antisense primer CZAP-AP (5 - ATATAGGCGGCCGCCCTCTGGACCTCTTCTCTTC-3 ). The sense primer lies upstream from an internal NheI site in NZAP; the antisense primer introduces a NotI site immediately downstream from the coding sequence to facilitate its cloning into the myc-tagged expression vector.

origin :

pi :

alt_names

rZAP

Zinc-finger Antiviral Protein

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARTD13, PARP13, Zap
gene	Zc3hav1
id	252832

genbank_ids

mutation

name

ZAP

shRNA_sequence

size

species

10116

Rattus norvegicus