pEAT8-137 M1V | Addgene 173807 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEAT8-137 M1V

catalog :

173807

citations: 1

Reference
Krishnan B, Hedstrom L, Hebert D, Gierasch L, Gershenson A. Expression and Purification of Active Recombinant Human Alpha-1 Antitrypsin (AAT) from Escherichia coli. Methods Mol Biol. 2017;1639:195-209 pubmed publisher

product information

Catalog Number :

173807

Product Name :

pEAT8-137 M1V

article :

doi	10.1007/978-1-4939-7163-3_19
id	28219986
pubmed_id	28752459

bacterial resistance :

Ampicillin

cloning :

backbone

pET-8C

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

For details on mutating an internal initiation site in the SERPIINA1 gene (changing the pEAT8 plasmid to pEAT8-137) see: Laska, M. E. (2001) The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli, Thesis, Massachusetts Institute of Technology, Cambridge, MA

growth strain :

Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

growth temp :

E coli BL21(DE3) can be used for expression. Note that the temperature for protein induction for soluble expression is 30 C (see PubMed 28752459).

origin :

pi :

alt_names

alpha1-antitrypsin M1V

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Unknown
promoter	T7
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF
gene	SERPINA1
id	5265

genbank_ids

mutation

E1M Otherwise this plasmid encodes the most common wildtype form of human alpha1-antitrypsin called M1V. The sequence is that of the mature protien (no signal seqquence) in UniProt entry P01009

name

SERPiNA1

shRNA_sequence

size

species

9606

Homo sapiens