This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-Sac-A (M1-42/C18C33)
catalog :
173759
citations: 1
Reference
Zhang S, Yoo S, Snyder D, Katz B, Henrickson A, Demeler B, et al. A Disulfide-Stabilized Aβ that Forms Dimers but Does Not Form Fibrils. Biochemistry. 2022;61:252-264 pubmed publisher
product information
Catalog Number :
173759
Product Name :
pET-Sac-A (M1-42/C18C33)
article :
doi10.1021/acs.biochem.1c00739
id28219977
pubmed_id35080857
bacterial resistance :
Ampicillin
cloning :
backbonepET vector
backbone_mutationVector is a modified pET vector. Restriction digest sites for Abeta gene are Nde1 (5') and Sac1 (3')
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses the A C18C33 peptide.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Sac1
cloning_site_5Nde1
promoterT7
sequencing_primer_35'- GTG GGT TCT AAC AAG GGT GCT ATC ATC TGC CTG ATG GTT GGT GGC GTT GTG ATC GCT -3'
sequencing_primer_55'- ATG GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG TGC -3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4
geneAPP
id351
genbank_ids
mutationchanged valine 18 to cysteine; changed glycine 33 to cysteine.
nameA (M1-42/C18C33)
shRNA_sequence
size156
species
9606
Homo sapiens
tags
resistance markers :
4486
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA