This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDisCoTune
catalog :
173713
citations: 1
Reference
Bertelsen A, Hackney C, Bayer C, Kjelgaard L, Rennig M, Christensen B, et al. DisCoTune: versatile auxiliary plasmids for the production of disulphide-containing proteins and peptides in the E. coli T7 system. Microb Biotechnol. 2021;14:2566-2580 pubmed publisher
product information
Catalog Number :
173713
Product Name :
pDisCoTune
article :
doi10.1111/1751-7915.13895
id28219967
pubmed_id34405535
bacterial resistance :
Chloramphenicol
cloning :
backbonepDisCoTune
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.
growth temp :
0.5 % Glucose
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5gccagcccaaatgcaatc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGR029W
geneERV1
id852916
genbank_ids
mutation
nameERV1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
alt_names
protein disulfide-isomerase
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5ACTTCCAGTATGTTACCGGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namePDI
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
3402
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA