This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHA-eIF4E
catalog :
17343
citations: 7
Reference
Fedder Semmes K, Appel B. The Akt-mTOR Pathway Drives Myelin Sheath Growth by Regulating Cap-Dependent Translation. J Neurosci. 2021;41:8532-8544 pubmed publisher
Majeed S, Batool A, Majeed R, Bhat N, Zargar M, Andrabi K. mTORC1 induces eukaryotic translation initiation factor 4E interaction with TOS-S6 kinase 1 and its activation. Cell Cycle. 2021;20:839-854 pubmed publisher
Knight J, Alexandrou C, Skalka G, Vlahov N, Pennel K, Officer L, et al. MNK inhibition sensitizes KRAS-mutant colorectal cancer to mTORC1 inhibition by reducing eIF4E phosphorylation and c-MYC expression. Cancer Discov. 2020;: pubmed publisher
Lock R, Ingraham R, Maertens O, Miller A, Weledji N, Legius E, et al. Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers. J Clin Invest. 2016;126:2181-90 pubmed publisher
Shi Y, Frost P, Hoang B, Yang Y, Bardeleben C, Gera J, et al. MNK1-induced eIF-4E phosphorylation in myeloma cells: a pathway mediating IL-6-induced expansion and expression of genes involved in metabolic and proteotoxic responses. PLoS ONE. 2014;9:e94011 pubmed publisher
Wong R, Hagen T. Mechanistic target of rapamycin (mTOR) dependent regulation of thioredoxin interacting protein (TXNIP) transcription in hypoxia. Biochem Biophys Res Commun. 2013;433:40-6 pubmed publisher
Okumura F, Zou W, Zhang D. ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP. Genes Dev. 2007;21:255-60 pubmed
product information
Catalog Number :
17343
Product Name :
pHA-eIF4E
article :
doi10.1101/gad.1521607
id1935
pubmed_id17289916
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-HA
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
RT-PCR product of mRNA from human A549 cell.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5KpnI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E
geneEIF4E
id1977
genbank_ids
NM_001968
mutation
nameeIF4E
shRNA_sequence
size650
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
resistance markers :
610
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA